The College of Education for Pure Sciences discussed a master dissertation on the isolation, morphological, and molecular identification of bacteria isolated from burns and evaluated the inhibitory effect of nano-zinc oxide and dandelion extract on Staphylococcus aureus by the postgraduate student, Ms. Dhafaf Mustafa Kamel.
The dissertation aimed at isolating and identifying Staphylococcus aureus bacteria from clinical samples of burn patients using traditional methods and automated confirmation, as well as determining antibiotic susceptibility patterns, classify multidrug-resistant isolates, and perform molecular detection of the mec, ica, and crt genes using PCR technology.
The dissertation demonstrated that Staphylococcus aureus is one of the most common pathogens in the samples studied, reflecting its clinical importance and its major role in causing infections.
The dissertation indicated that phenotypic and biochemical tests supported by molecular diagnostics using polymerase chain reaction (PCR) technology proved highly efficient in accurately identifying the isolates, and that the detection of resistance-associated genes (mec, crt, ica) revealed the presence of antibiotic-resistant strains.
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