The College of Science, Wasit University discussed a master dissertation on the integrated evaluation of vegetatively synthesized silver nanoparticles and plant aqueous extracts against clinical Malassezia isolates by the postgraduate student, Mr. Ali Dhia Salman.

The dissertation reviewed the green synthesis and physicochemical characterization of silver nanoparticles (AgNPs) using plant extracts (Ziziphus spina-christi and neem).

The dissertation demonstrated determining the minimum inhibitory concentrations (MIC) and minimum lethal concentrations (MFC) of the extracts and nanoparticles against Malassezia spp. fungi, comparing their antifungal performance with that of conventional drugs (itraconazole and fluconazole). 

The dissertation highlighted the dose-dependent inhibitory effect using agar diffusion techniques, and measured the effect of treatment on virulence genes (lipase gene) using real-time polymerase chain reaction (qRT-PCR) technology, as well as studying the epidemiological correlations between infection and demographic variables (age, gender, occupation, contact with animals) in Wasit Governorate.